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Spontaneous Formation of Datrizoic Acid-Containing Dendrimer-Stabilized Gold Nanoparticles for Enhanced Computed Tomography Imaging Applications

Chen Peng, Xueyan Cao, Rui Guo, Tingting Xiao, Mingwu Shen, Xiangyang Shi*

 

Abstract: We report a novel approach to preparing dendrimer-stabilized gold nanoparticles (Au DSNPs) through the use of amine-terminated fifth-generation poly(amidoamine) (PAMAM) dendrimers modified by diatrizoic acid (DTA) as stabilizers. This approach allows the spontaneous formation of Au DSNPs via reduction of gold salt by the dendrimer itself in aqueous solution at room temperature. Further acetylation of the remaining terminal amino groups of the dendrimers enables the generation of Au DSNPs with improved biocompatibility. We show that the formed {(Au0)50-G5.NHAc-DTA} DSNPs with an average diameter of 6 nm are very stable in aqueous solution. In vitro 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay reveals that the formed Au DSNPs do not display cytotoxicity at a concentration up to 3 M. X-ray absorption coefficient measurements show that the DTA-containing Au DSNPs have enhanced attenuation intensity and can be used for CT imaging of cancer cells in vitro. With the two radiodense elements of Au and iodine incorporated within one particle and good biocompatibility, the formed {(Au0)50-G5.NHAc-DTA} DSNPs with enhanced X-ray attenuation property should be applicable for CT imaging of various biological systems.

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