NMLONLINE.ORG

YANG

In vitro evaluation of pH-sensitive doxorubicin nanoliposomes modified with carboxymethyl chitosan

Shanxiang Yang, Xiuzhen Qian*, Yunlong Xu*, Xinyi Tao, Chongjun Zhao

 

Abstract

The property in vitro of pH-sensitive doxorubicin nanoliposomes modified with carboxymethyl chitosan (CMCS-DOX-NL) was investigated. CMCS-DOX-NL had an encapsulation efficiencies of (88.8 ± 0.84)% and an average particle size of (21.7±10) nm. The drug release in vitro of the CMCT-modified DOX nanoliposomes showed obvious pH sensitivity. Cells viability assay showed that cytotoxicity of CMCS-DOX-NL on HepG-2 cells was less than that of the common DOX nanoliposomes (DOX-NL). The results for the flow cytometry analysis and fluorescence microscopy micrographs suggested that the cells treated with CMCS-DOX-NL exhibited significant increase in cellular association or DOX uptake.

Fulltext: PDF



You are here: